U2OS cells expressing mOrange2-SEC23, LAMP1-HaloTag, mEmerald-ALG2, or mEmerald-ALG2 E47A/E114A mutant were treated with 250 nM Torin for 6 h. The images show before Torin treatment (upper), after Torin treatment (middle), or in cells expressing the ALG2 mutant after Torin treatment (bottom). Arrows point to an ALG2 signal colocalized with a SEC23-labeled ERES and in contact with a LAMP1-positive structure. Scale bar: 1 um.
