Quantification of the percentage of SEC23 colocalized with LAMP1-labeled structures (upper graph), SEC23 colocalized with LC3-labeled structures (middle graph), or LAMP1-labeled structures colocalized with LC3-labeled structures (lower graph) in cells that were untreated, treated with Torin, Torin plus wortmannin, or Torin plus bafilomycin A1 for 6 h. One-way ANOVA,****p < 0.0001; ns, non-significant.