Probability density of LD song scores (averaged within each individual) of translocated males with 100% Dutch ancestry (purple, n= 141 songs, 7 individuals) when projected onto the LDA model trained on songs of unmanipulated Dutch (red, n= 267 songs, 26 individuals) and Swedish (blue, n= 361 songs, 38 individuals) birds. Boxplots show median, quartiles, and quartiles +- 1.5*IQR. Each point represents the mean LD score of songs per individual. Horizontal and vertical jitter was added to the points to reduce overlap. Letters (a-c) above the LD distribution of each experimental group denote significant differences between the groups. p values were extracted from post hoc tests performed on the linear mixed model. Plot of individual variation in LD song scores of translocated males is presented in Figure S1. See Tables S2 and S3 for analysis of temporal and spectral parameters contributing to song differences between the experimental groups.