Midguts stained with anti-pH3 (red), anti-GFP (green; driven by the esg-Gal4 in EGT), and DAPI (blue). The GFP labels ISCs and their immediate progeny. The white arrowheads highlight examples of pH3-positive cells. The flies were fed normal food for 3 days and then food laced with 30 mM CAN for 2 days. Scale bar: 50 μm.