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Relative LC-MS/MS quantification of octopamine in heads of D. melanogaster wild-type stocks (reds) and D. mojavensis subspecies (blues). Data represent two independent experiments, each with three biological replicates per group (n= ~100 heads/biological replicate; squares represent data from experiment #1, triangles are from experiment #2). One-way ANOVAs find a significant effect of genotype for octopamine (F(5,30)= 9.488,p< 0.0001).
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