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Immunoblot analysis of HEK293ET cells transiently transfected with plasmids encoding the indicated GFP-tagged effector and infected with the Bt E264::tssMpknockstrain. Mock transfected cells (mock TF), refers to cells that did not receive DNA. Values show mean of three biological repeats ± SEM (B and E). Other data are representative of at least three biological repeats. Statistical significance was assessed by two-way ANOVA with Sidak’s multiple comparisons test;****<0.0001. See alsoFigure S1.

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