Antibiotic susceptibility in permeabilized bladders. 8-week-old female CBA/J mice were infected with 108of the UTI89+TM strain and sacrificed at day 2 p.i.n= 8 bladders were homogenized in saline + 0.025% Triton X-100 to permeabilize the bladder urothelium. Initial total CFU including the fraction of dividing cells were determined (control). These homogenates were then treated with ciprofloxacin or delafloxacin for 4 h, and CFUs were determined (+cipro+triton and +delaflo+triton). Separately, 16 (n= 8 andn= 8) bladders were treated with ciprofloxacin and delafloxacin respectively for 4 h before homogenization (+cipro and +delaflo). Each dot represents one bladder; lines are means and bars are SEM. Black dotted lines represent the limit of detection (1 colony per 100 L). Significance was determined using the Kruskal-Wallis test with adjustment for multiple comparisons. Allpvalues are shown; statistically significantpvalues (<0.05) are in red. Data are from one experiment with 8 mice per group. Blue dots represent LacZ+ fraction > 75%, gray dots LacZ+ fraction < 25%, and mixed dots LacZ+ fraction between 25% and 75%.
