Line Plot from Scientific Research

Plots displaying metagene analysis of ribosome profiling and RNA-seq reads. Coverage by ribosomes was calculated using the midpoint of the ribosome-protected fragments as a pseudo A-site. For RNA-seq reads, coverage across the entire mapped fragment was utilized. Coverage at each position in genes with 5 UTR, CDS, and 3 UTR of at least 100 nt in length were normalized by the mean coverage across the whole gene. Normalized coverages were then separated by the feature they mapped to, distributed evenly across 100 bins, and averaged across all analyzed genes. Relative normalized reads were plotted against a model gene 300 nt in length, with the first 100 nt representing the 5 UTR and the last 100 nt representing the 3 UTR. The dashed vertical lines indicate the start and stop of the CDS in the model gene, respectively.

DOI