Quantification of nuclear H2A.x foci from S phase SH-EP cells treated for 24 h with and without Dox (1 ug/mL) to induce MYCNwtor MYCN3A. Cells were treated for 4 h with indicated topoisomerase inhibitors (doxorubicin 250 nM; irinotecan 25 uM; etoposide 2.5 uM). Shown are single-cell analyses containing all biological replicates. pvalues calculated with two-sided Wilcoxon rank-sum tests (n= 4 for DMSO; n= 2 for all other samples).
