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(Left) The first 160 amino acids (aa) of MYCN were displayed as arrayed 72 sequential 20mers with an overlap of 18 aa and an offset of 2 aa. Arrays were incubated with Cy5-labeled RNA 62 nt. Shown are theZscores of fluorescence intensities for all tested peptides. The oligopeptide with the strongest RNA binding, its sequence, and the critical amino acids are highlighted. Data shown as mean ± SEM (n= 6). (Right) Same procedure, but for the last 92 aa of MYCN using sequential 25mers with an overlap of 23 aa and an offset of 2 aa. Data shown as mean ± SEM (n= 3). MYCN protein domains shown above the plots.
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