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Cell lysates and anti-hemagglutinin (HA) immunoprecipitates were blotted for endogenous ADAM17, HA (iRhom2), and actin. In (E), EV and WT conditions are on the same immunoblot as mutant conditions, with superfluous lanes removed to make comparison easier.*indicates non-specific signal. Data are representative of three independent experiments (B–F). See alsoFigures S3andS4anTable S1.

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