Bar Plot from Scientific Research

Immunoblot images (left) and quantification (right) of PCK1, pAMPKThr172, AMPK, pAktS473, Akt, PPARα, PDK4, and VINCULIN in liver homogenates of mice fed a WD. VINCULIN served as a loading control for PPARα. PCK1, pAMPKThr172, AMPK, pAktS473, Akt, and PDK4 were run on separate blots; therefore, VINCULIN served as a sample preparation control for these blots. n= 3 for all conditions. Data are expressed as mean ± SEM. Statistical significance was calculated using two-way analysis of variance with post hoc Tukey’s test (B–G and I) or Student’s test (B–D; fold change). ∗p< 0.05,∗∗p< 0.01,∗∗∗p< 0.001, and∗∗∗∗p< 0.0001. NS, non-significant.

DOI