Line Plot from Scientific Research

In vitro dsDNA cleavage activity of wild-type (WT-)GeoCas9 and iGeoCas9, determined by denaturing PAGE (n= 3, data are represented as mean SD). MgCl2 concentration indicated above the graph. Fractions were collected at 0 s, 30 s, 1 min, 2.5 min, 5 min, 10 min, 30 min, 1 h, and 2 h. Fraction 0 is represented by the substrate only. The rate constants kobs are listed in the sample legend. See also Figure S4C.

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