Line Plot from Scientific Research

In vitrodsDNA cleavage activity of wild-type (WT-)GeoCas9, iGeoCas9, GeoCas9(R1), and GeoCas9(KGR) determined by denaturing PAGE (n= 3, data are represented as mean ± SD). Substrate and PAM sequences are indicated above the graph. Fractions were collected at 0 s, 30 s, 1 min, 2.5 min, 5 min, 10 min, 30 min, 1 h, and 2 h. Fraction 0 is represented by the substrate only. The kobs for each Cas9 are listed in the sample legend. See also Figure S4B.

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