Protein Structure from Scientific Research

Comparison of wild-type GeoCas9 and iGeoCas9 at WED domain amino acid positions 908, 843, and 884. Positions 908 and 843 are represented by an EM density map (gray) and model (sticks and ribbons). PAM nucleotide positions on the non-target strand are labeled in descending from 1 to 8 in the 5 to 3 direction. Nucleotides on the target strand are assigned the same number as their complementary nucleotides on the non-target strand. Potential DNA-protein interactions are indicated with a dashed line and atomic distance. Amino acid position 884 is represented by a Coulombic electrostatic surface potential map and encircled with a dashed line (red, negative; blue, positive; white, non-polar). NTS, non-target strand; TS, target strand; PAM, protospacer adjacent motif. See alsoFigures S1S3andTable S1.

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