Line Plot from Scientific Research

H2 gas production monitored from cell lysates in E. coli BL21(DE3) cells expressing the Th1 and Th2 [FeFe] hydrogenases from archaea. The cell lysates were activated by addition of [2Fe]adt. H2 levels were measured every 15 min for 2 h by gas chromatography after addition of methyl viologen and dithionite to activated cell lysates, set to pH 6.8 with 100 mM KPi buffer. Activities are normalized for number of cells used (nmol H2OD6001) and error bars reflect standard deviations from two biological triplicates. Blank represents the same strain but contains an empty vector. See also Figures S1S4 and S7.

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