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H2gas production monitored from cell lysates inE. coliBL21(DE3) cells expressing group A1, B, and E [FeFe] hydrogenases from archaea. All cell lysates, including the blank, were activated by addition of [2Fe]adt. H2was measured by gas chromatography (GC) after addition of methyl viologen and dithionite to activated cell lysates, set to pH 6.8 with 100 mM KPi buffer. Activities are normalized for number of cells used (nmol H2min-1OD600-1) and error bars reflect standard deviation from biological triplicates. The strain expressing prototypicalCrHydA1 was used as a positive control while blank represents the same strain but containing an empty vector.

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