Group E [FeFe] hydrogenase from Ca.Forterrea multitransposorum. For each panel, the catalytic domain (H-cluster), iron-sulfur binding motifs, and amino acid sequence length are shown at the top. Genes encoding hydrogenase structural subunits are shown in their genetic context beneath, labeled and colored consistent with the corresponding subunit in the structural models. Predicted cofactors are positioned based on the structures of homologous proteins. A zoomed view of the H-cluster and conserved coordinating cysteine residues (C1to C5) is shown for each group. hydA, [FeFe] hydrogenase;hydB, diaphorase;hydC, thioredoxin;hydD,nuoG-like conduit protein;hyd6TM, uncharacterized 4- to 6-helix transmembrane protein associated with group A [FeFe] hydrogenases; (His)[4Fe4S], (Cys)3His-ligated [4Fe4S] cluster binding domain; [2Fe2S], [2Fe2S] cluster binding domain; [4Fe4S], [4Fe4S] cluster binding domain; 2[4Fe4S], bacterial ferredoxin-like 2[4Fe4S] cluster binding domain; 6Cys, putative iron-sulfur cluster binding domain.*HydC protein in group A1 gene cluster was not predicted to form a complex with HydA. Surface structures are used for the multisubunit group B and A3 [FeFe] hydrogenases, with ribbon diagram versions provided inFigure S3. See alsoFigures S1S4.
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