Representative images of immunofluorescence staining of ABCG2 and basolateral marker Na/KATPase. The white arrow heads indicate the distribution of ABCG2 (red). In WT/WT mice, ABCG2 is distributed to the apical side just beneath the LTL signal. However, in NUMB R632H /NUMB R632H mice, ABCG2 located to the basolateral side with increased co-localization with Na/K ATPase. The illustration of ABCG2 distribution is shown in the bottom left, and the statistic result of the percentage of apical ABCG2 is shown in the bottom right ( n = 6 per group, t = 19.34, df = 10). Statistics was calculated using two-tailed unpaired Student’s t test. The results are presented as mean ± SD.
