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Expression of NUMB WT and NUMB R630H protein. WT NUMB-3FLAG or NUMB R630H -3FLAG plasmid was co-transfected with a vector expressing GFP into HEK293 cells, followed by detecting NUMB WT -FLAG and NUMB R630H -FLAG proteins. GFP and beta-ACTIN were detected as the transfection and loading control, respectively. The detection was repeated three times. The statistic result is shown in the bottom panel ( n = 3 per group).
#Bar Plot#Photo#Gel#NUMB WT#NUMB R630H#Protein Expression#HEK293 Cells#GFP#beta-ACTIN#Transfection#Loading Control
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