Expression of NUMB WT and NUMB R630H protein. WT NUMB-3FLAG or NUMB R630H -3FLAG plasmid was co-transfected with a vector expressing GFP into HEK293 cells, followed by detecting NUMB WT -FLAG and NUMB R630H -FLAG proteins. GFP and beta-ACTIN were detected as the transfection and loading control, respectively. The detection was repeated three times. The statistic result is shown in the bottom panel ( n = 3 per group).
