Co-immunoprecipitation of NUMB and ABCG2. ABCG2-FLAG plasmid or empty vector was transient-transfected into HEK293 cells. Immunoprecipitation was done with FLAG antibody, followed by detecting the co-precipitation of ABCG2-FLAG with endogenous NUMB and ABCG2-FLAG and NUMB in input b . Star indicates non-specific band.
