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IFN-γ-secreting T cells were assessed using an ELISpot assay. PBMCs isolated from HPV16 + CESC patients or healthy donors (HD) were co-incubated with hEry, MHC-I-E7-hEry, or HPV16 E7 peptide (YMLDLQPET) for 48 h. Left: representative images of ELISpot wells. Right: the quantification of IFN-γ spots (CESC patients, n = 8; HD, n = 4).
#Scatter Plot#Box Plot#Photo#Microscopy#IFN-γ#T Cells#ELISpot Assay#PBMCs#HPV16 + CESC Patients#Healthy Donors#hEry#MHC-I-E7-hEry#HPV16 E7 Peptide#IFN-γ Spots
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