Confocal images ( d and f ) and quantification ( e and g ) of c-Fos-expressing neurons in the mPFC ( d and e ) and NAc ( f and g ) for the same conditions as in b and c . Scale bars, 100 m ( d and f ). In c , e and g , statistical significance was tested via one-way ANOVA and Tukey’s multiple comparison tests ( n = 6).