(i) Tangent modulus as a function of strain for ramps at 1% s -1 for WT (orange) and K14-R125C (blue) monolayers. (ii) Box plots of the tangent modulus at low strain ( L 15%) and at high strain ( H 70%) in WT and K14-R125C monolayers. Fold change is indicated between the box plots. P = 0.007 and P = 0.96 for WT and K14-125 monolayers, respectively. The solid lines represent the average value and the shaded areas, the standard deviation. In the box plots, the central mark indicates the median, and the bottom and top of the box indicate the 25th and 75th percentiles, respectively. The whiskers extend to the most extreme data points that are not outliers. The data points are indicated by grey dots and the outliers, by red ‘+’ symbols. Statistics: two-sided Kolmogorov–Smirnov test, P < 0.01. Data from n = 10 WT, n = 9 K14-R125C, n = 11 NS and n = 12 DSP-shRNA monolayers.
