Representative confocal images of a monolayer profile during calyculin treatment. Intercellular junctions are visualized with E-cadherin-GFP (green) and cells are visualized by dye exclusion of dextran-Alexa 647 (red) added to the medium. Scale bars, 10 m. (i) Profile view over time. Time is indicated in the bottom-left corner of each image. (ii) XY view of a defect in a monolayer and its corresponding XZ and YZ profile views. The dashed yellow lines indicate the position of the profile views. Time is indicated in the bottom-left corner.
