Volcano plot of differential gene expression analysis (two-sided Wilcoxon test, Benjamini–Hochberg correction) between malignant cells residing close to a T/NK cell and those that do not across all biopsies, measured by cell-segmented MERFISH data. Genes are colored by their directionality in the sample-specific differential expression analysis. Genes with different directionality between patient-specific and combined analysis show discordant coloring. FC, fold change; man, manual.