Competition flow cytometry assays in PC9 MLH1 cells comparing the growth of NT gRNA GFP cells with epegRNA BFP cells harboring different EGFR variants in the presence and absence of osimertinib (75 nM) for 5 days. Data are normalized to day 0 ratios and represent the mean s.d. of biological triplicates. Unpaired, two-tailed Student’s t -test comparing with the EGFR C797C synonymous variant control; * P = 0.0003, ** P = 0.0002, *** p < 0.0001. Predicted amino acid editing consequences are labeled for drug resistance variant screens. See also Extended Data Fig. 5 .
