Microscopy from Scientific Research

β-galactosidase staining for senescent cells; β-galactosidase positive senescent foci (blue) are indicated with arrows. HT-29 cells were treated with the combination of dabrafenib (80 nM) and cetuximab (1 µg ml -1 ) or DMSO as a control for 48 h before analysis. Representative images are shown for the drug addiction variant MAP2K1 Y130C. Scale bar, 500 m. Predicted amino acid editing consequences are labeled for drug resistance screens and genotyped edits are shown in d , e and f . Data are the average of two independent experiments performed on separate days, or representative of two independent experiments ( e and f ). See also Extended Data Figs. 2 and 3 .

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