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Codelivery of iGeoCas9 RNPs and ssDNA HDR templates with LNPs edits the chromophore of EGFP to BFP in HEK293T cells. Top, target and donor designs for iGeoCas9-mediated chromophore editing. Bottom, genome-editing efficiencies quantified based on EGFP and BFP signals using iGeoCas9 paired with different sgRNAs plus or minus ssDNA templates. iGeoCas9 RNP with ssDNA generates between 20% and 40% HDR in HEK293T cells. GFP, green fluorescent protein.

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