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Schematic diagram of GeoCas9-mediated genome editing of NPCs isolated from Ai9 mice. The spacer and PAM sequences of the GeoCas9 gRNAs were designed to turn tdTomato if successful editing occurs. Guides g7 and g8 target the LoxP sites for the stop cassette deletion. DSB, double-strand break.
#Workflow#Illustration#Table Image#GeoCas9#Genome Editing#NPCs#Ai9 Mice#Spacer Sequence#PAM Sequence#gRNAs#tdTomato#LoxP Sites#Stop Cassette Deletion#Double-Strand Break
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