Protein Structure from Scientific Research

The reconstruction refined from combined images, generated by subtracting the ADF images from the EBF images. The volume shown in a resolves the most features of worm hemoglobin, showing a better-quality reconstruction than b or c and a higher-resolution map-to-model FSC (PDB- 5M3L ) 25 at a 0.5 cutoff. The SPA reconstructions of WH were refined from the same subset of 3,086 particles, extracted from the different images. The scale bar corresponds to 10 nm.

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