Line Step from Scientific Research

Data filtration techniques implemented by CyLinter for the removal of bright nuclei (as demonstrated in TOPACIO, specimen 110). The top plots show density histograms of mean Hoechst signal for cells in the given tissue. The bottom images show Hoechst signal (colormap) in a region of the same tissue with cells falling within the green region in the above histogram highlighted by green points. Nuclear segmentation outlines are shown for reference in all cases (translucent outlines). Note that, unlike h k, which highlight cells to be excluded from analysis, cells highlighted in l are to be retained for further analysis.

DOI