Number of identified proteoforms when combining multiple database search results from the different approaches. All raw files were analyzed together in a multiconsensus analysis, and the number of proteoforms identified in a combination of various sample preparations was calculated. MWCO, 30-kDa filter; ACN, acidic (TFA/NaCl) ACN depletion; SPE, C18 material; PEPPI with subsequent MCW precipitation.
