Number of identified proteoforms (a) and protein accessions (b) (n = 3 replicates of independently performed sample preparations, average plusmn standard derivation). Full-length proteoforms are those deposited in the proteoform database, including start methionine excision and previously described proteolytic-processed proteoforms, for example, with cleaved signal peptides. By contrast, truncated proteoforms refer to all subsequence proteoforms not defined in the database.