Microscopy from Scientific Research

To measure resolution, we used block-wise FRC resolution analysis 14 . The method requires more than one independent image of the same region for noise realization. Left and middle, two independent confocal images (single xy plane) of expanded HEK293 cells with pre-expansion microtubule staining, showing the same region of interest under the same imaging conditions. Right, local mapping of FRC resolution values. A global FRC resolution is calculated by averaging FRC resolution values across all blocks.

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