Microscopy from Scientific Research

Open access visualization of Microscopy, POLCAM SMOLM Reconstruction, Intensity-only Algorithm, Asymmetric Gaussian Fitting, DSF-fitting Algorithm
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A POLCAM SMOLM reconstruction of a 200 × 200-pixel region from Fig. 4 with 10,000 frames, as analyzed by the intensity-only algorithm (with rotated asymmetric Gaussian fitting) and the DSF-fitting algorithm. Both reconstructions were rendered using approximately the same number of localizations (258,251 localizations for the intensity-only algorithm, 258,172 localizations for the DSF-fitting algorithm).

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