Line Plot from Scientific Research

The bias on the estimation of the out-of-plane angle θ as a function of rotational mobility γ for the intensity-only algorithm, determined using simulated images of single dipole emitters (using 1,000 photons per emitter and ten background photons per pixel). A rotational mobility parameter γ of 0 corresponds to total rotational freedom, and a value of 1 corresponds to perfect rotational immobilization.

DOI