Microscopy from Scientific Research

Simulated examples of four single fluorescent molecules. From top to bottom, a molecule aligned with the x axis (first row), the y axis (second row), the optical axis (z axis) (third row) and a rapidly rotating molecule (fourth row). For each example, the following is shown: the image plane recorded with a regular monochrome camera and the image plane recorded with a polarization camera in its raw format and in a format where the pixels have been rearranged to form four images that are each made up only of pixels that are covered by a micropolarizer with the same transmission axis orientation.

DOI