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Naive WT and Hif1alpha -/- CD4 + T cells were activated under normoxia (21% O 2 ) and hypoxia (1% O 2 ) for 5 days. Gated live cells were analyzed for the expression of ICOS and CD25, depicted as geometric mean fluorescence intensity (gMFI). N = 4 per group, **** p < 0.0001, *** p = 0.0001.
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