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mRNA expression of glycolytic genes was evaluated by real-time RT-PCR in T cells activated and cultured under normoxia (21%) and hypoxia (1%) for 48 h ( N = 6, ** p = 0.0029 for Gpi , ** p = 0.0053 for Pkm2 , *** p = 0.0007 for Hk2, * ** p = 0.0009 for Ldha, and **** p < 0.0001 for Mct4 by Two-way ANOVA with dak’s multiple comparisons test (with adjustment))
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