Further validation of the top-combinations for the 4 patient samples using population-level flow cytometry assays in the same patient-derived cells. Toxic effects (left-hand bars) scored based on co-inhibition of normal cell populations, and therapeutic effects (right-hand bars) based on co-inhibition of malignant cells. The predicted effective doses are indicated in parentheses (μM), and the dotted vertical lines indicate 50% inhibition level. n, the number of replicate screens. Statistically significant differences in combinatorial responses between cancerous and normal cells were observed, with p < 0.01, < 0.05, and < 0.05 for patients 5, 6, and 11, as assessed with the two-sided Wilcoxon signed-rank test. For patient 12, given the limited number of biological replicates ( n = 2), statistical significance cannot be tested. The error bars represent the standard error of the mean (SEM). Box plots show the median (central line), 25th and 75th percentiles (box edges), and the range within 1.5 times the interquartile range from the box (whiskers). Source data are provided as a Source Data file.
