All the model-predicted drug combinations exhibited either synergistic (ZIP > 10) or additive effects (0 < ZIP < 10) in the whole-well combinatorial viability assay. Statistical analysis showed significant effects (p < 0.001, two-sided Wilcoxon test; upper panel), when comparing measured ZIP scores to zero (no effect). The assay involved n = 24 combinations for Patients 5 and 6, n = 11 combinations for Patient 11, and n = 20 for Patient 12, with each patient’s results representing technical replicates derived from different drug combinations tested on cells from the respective patients. Two examples of combinations with ZIP = 13.6 and ZIP = 13.5 as tested in multi-dose drug combination assays (lower panel). Interactive plots of the dose-response matrices for all the predicted combinations are provided at https://ianevskialeksandr.github.io/waterfall_plot.html . Scale bars represent percentage inhibition from 0 to 100 (white to red).
