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Pull-down experiments with symmetric and H3X/H3Y-based asymmetric H3K27me3 nucleosomes and 500, 100, 25, and 5 μg of nuclear extract. Shown are representative blots and quantification from three independent experiments. Plots show the mean and SD of the ratio of binding to symmetric and asymmetric nucleosomes, normalized to the ratio at 500 μg of nuclear extract. See alsoFigures S5andS6andTable S4.
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