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Pull-down experiments with recombinant H3K4me3-binding TAF3 PHD domain (top) and H3K27me3-binding CBX7 chromodomain (bottom), detected via anti-His antibody. Asymmetric nucleosomes were generated with the histone H3X/H3Y system. Shown are representative blots and quantification (mean and SD of background-corrected band intensities) from three independent experiments.

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