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The induction of a two-color dual-reporter system sensitive to inhibitors of ribosome progression or of DNA replication. Induction of the expression of Katushka2S (red color) is triggered by translation inhibitors, whereas RFP expression (green pseudocolor) is induced upon DNA damage. Control antibiotics levofloxacin (Lev) and erythromycin (Ery), and tested PrAMPs were placed on agar plate containing E. coli BW25113 Δ tolC cells transformed with the pDualrep2 plasmid 57 .

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