Line Plot from Scientific Research
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SBP-tagged REEP1 (REEP1-SBP) was expressed alone or with FLAG-tagged ATL1 (ATL1-FLAG) in Expi293 cells and purified by digitonin detergent-solubilization and binding to streptavidin- (REEP1-SBP) or anti-FLAG (ATL1-FLAG/REEP1-SBP) beads. Shown is the elution profile of the samples subjected to size exclusion chromatoography and the corresponding fractions as analyzed by SDS-PAGE and Coomassie blue staining. The asterisk corresponds to the 3xFLAG peptide used for ATL1-FLAG elution.
#Line Plot#Gel#REEP1-SBP#ATL1-FLAG#Expi293 Cells#Digitonin#Streptavidin Beads#Anti-FLAG Beads#Size Exclusion Chromatography#SDS-PAGE#Coomassie Blue Staining#Elution Profile
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