Microscopy from Scientific Research
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U2OS cells stably expressing the ER marker Sec61β fused to red fluorescent protein (RFP-Sec61β) were transfected with hemagglutinin (HA)-tagged REEP1 carrying the disease mutation Δ113-201, immunostained with anti-HA (αHA) antibodies, and imaged by confocal fluorescence microscopy. Right panel shows an overlay between αΗΑ (green) and RFP-Sec61β (magenta). Scale bar, 2 µm.
#Microscopy#U2OS Cells#ER Marker#RFP-Sec61β#Hemagglutinin#HA-tagged REEP1#Disease Mutation#Immunostained#Anti-HA Antibodies#Confocal Fluorescence Microscopy
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