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Endogenous REEP1 localization in SaOS2 cells stably expressing the ER marker Sec61beta fused to near-infrared fluorescent protein (iRFP-Sec61beta). Cells were analyzed by indirect immunofluorescence using anti-REEP1 (alphaREEP1) and anti-alpha tubulin (alphatubulin) antibodies, and imaged using confocal fluorescence microscopy. Shown are whole cells and enlargements of the boxed region. Right column shows overlays of alphaREEP1 (green) and iRFP-Sec61beta (magenta). Bottom two rows show cells after 50 μM nocodazole treatment.
#Microscopy#Endogenous REEP1 Localization#SaOS2 Cells#ER Marker#Sec61beta#Near-Infrared Fluorescent Protein#iRFP-Sec61beta#Indirect Immunofluorescence#Anti-REEP1 Antibodies#Anti-Alpha Tubulin Antibodies#Confocal Fluorescence Microscopy#Nocodazole Treatment
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