Schematic representation of the regions of Tse15 fused to the C-terminal end of the T25 adenylate cyclase fragment. Each was tested for their ability to interact with one or more regions of VgrG15 fused to the C-terminal end of the T18 adenylate cyclase fragment (text at right). Region of Tse15 and derivatives shaded orange represents the clade domain, grey regions represent the Rhs domain and blue region (in Tse15 wildtype only) represents the toxin. Numbers indicate amino acid residues of wildtype Tse15 or VgrG15 each fragment represents. Interaction between a T25 fusion proteins and a T18 fusion protein is indicated with a +, no interaction is indicated by a dash. An example of a positive and negative colony can be seen in Fig. 4c .
