Huh7 cells, with or without endogenous SUCLA2 depletion and reconstituted expression of WT rSUCLA2 or rSUCLA2 S124A, were cultured in a medium containing 5 mM β-HB and treated with or without IGF1 for 24 h. The concentrations of intracellular AcAc were measured. The data represent the average of three independent experiments (mean ± SD).**p< 0.01;***p< 0.001;****p< 0.0001.
